咽灵口服液对慢性咽炎家兔模型炎症因子影响研究.pdf

成都中医药大学硕士学位论文


中文摘要

目的:研究咽灵口服液对慢性咽炎家兔模型肿瘤坏死因子α(TNF-α)、血
管细胞粘附因子1(VCAM-1)的影响,并探讨其治疗慢性咽炎作用机制。
方法:依据导师彭顺林教授的“慢性咽炎造模方法”造模,三周后慢性咽炎
的动物模型成功,再随机分组:自然恢复组(简称模型组)、阳性对照组(简称
对照组)、咽灵口服液低剂量组(简称低剂量组)、咽灵口服液中剂量组(简称中
剂量组)、咽灵口服液大剂量组(简称高剂量组),分别给予咽灵口服液低、中、
高剂量灌胃治疗,阳性对照组用咽炎片、空白组用生理盐水灌胃,连续用药7天。
观察咽灵口服液对实验性慢性咽炎兔的一般情况、体温、白细胞(简称WBC)计
数、中性粒细胞百分比(N)、单核细胞百分比(M)及淋巴细胞百分比(L)的变
化,HE染色光镜观察局部组织病理形态学变化,用ELISA法检测家兔血清中肿瘤
坏死因子α(TNF-α)、血管细胞粘附因子1(VCAM-1)等细胞因子的含量变化。
结果:各组药物对慢性咽炎家兔的症状均有不同程度的改善;但以咽灵口
服液高剂量组最佳,低剂量组最差。其不但使家兔的一般情况改善,而且使家兔
升高的体温、白细胞、L、M、N降低;局部病理形态学得到改善,炎症细胞浸润
明显减少甚至消失;降低了升高的血清中肿瘤坏死因子α(TNF-α)、血管细胞粘
附因子1(VCAM-1)的含量。
结论:咽灵口服液中药复方滋阴润燥、活血化瘀、解毒利咽、标本兼治的
作用是通过抗炎、增强免疫、调节细胞因子、抑制组织增生等多途径、多环节发
挥作用来实现。这为提高中医药防治慢性咽炎提供了科学的依据和有价值的参
考。
关键词:咽灵口服液慢性咽炎细胞因子 TNF-α VCAM-1






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成都中医药大学硕士学位论文


ABSTRACT

Objection: To study the influence of Yanling oral liquid on chronic pharyngitis
of rabbits and provide experimental evidence on chronic pharyngitis treated.
Method: According to the method of professor Peng chronic pharyngitis of
rabbits induced models. Three weeks later, induced models eed. 60 Japanese big
ear rabbits were divided into 6 groups randomly including vacant group, model group,
controlled group, low dosage group, middle dosage group and high dosage group. The
rabbits had been getting ammonia and turpentine until they turned the animal model
with chronic pharyngitis. Seven days later the general condition and the index of the
laboratory examination were observed including temperature and WBC etc. AT the
same time we observed the morphological changes of pharyngeal mucous
membrane of rabbits by optic microscope after HE stained. In addition, we measured
the concentration of TNF-αand VCAM-1 by enzyme-linked immuno sorbent assay
(ELISA).
Result: The symptoms of the rabbits with chronic pharyngitis turn better in
different degrees,in which high dosage group is best and low dosage group is worst.
Yanling oral liquid de