Lecture 10 Bioengineering applications of hydrogels Molecular Imprinting and Drug Delivery.pdf

Molecular Principles of Biomaterials Spring 2003
Lecture 10: Bioengineering applications of hydrogels: Molecular
Imprinting and Drug Delivery
Last Day: polyelectrolyte gels
plexes and multilayers
Applications in bioengineering
Theory of ionic gel swelling
Today: Molecular imprinting
Hydrogels in drug delivery
Supplementary Reading: . Lustig and . Peppas, ‘Solute diffusion in swollen membranes. IX. Scaling laws
for solute diffusion in gels,’ J. Appl. Polym. Sci. 36, 735-747 (1988)
T. Canal and . Peppas, ‘Correlation between mesh size and equilibrium degree of
swelling of works,’ J. Biomed. Mater. Res. 23, 1183-1193 (1989)
Molecular Imprinting1,2
Concepts of molecular imprinting
• Molecular imprinting is the design of works that can recognize a given target molecule and bind it
preferentially in the presence of an excess of irrelevant molecules, some of which may have very similar
molecular structures
o Seeks to mimic specificity in biological recognition obtained through protein-protein interactions
• Steps to the preparation of molecularly-works:
1. mixing of binding monomers and target molecule
o target can be mixed directly with liquid monomers in bulk or co-dissolved in a
non-interfering solvent
o monomers bind target
ƒ covalent interactions
ƒ non-covalent bonding
ƒ metal coordination
o mixture usually at high concentration (. 50% w/vol solutions): enforces close
interactions of target with binding monomers and leads to a work that
holds the position of functional groups in position of template binding
2. polymerization of monomers in place
o usually photopolymerization (rapidly ‘trap’ structure)
3. washing for removal of target molecule work pockets
Lecture 10 – Bioengineering Applications of Hydrogels 1 of 12
Molecular Principles of Biomaterials Spring 2003
• types of target molecules:1
o small-molecule drugs
o steroids