表达新城疫病毒f、hn基因重组鸡痘病毒疫苗的遗传稳定性和免疫效力试验.pdf

扬州大学硕士学位论文定的保存期内,7种保护剂的保护效力差异不显著。为了评价这两种疫苗的免疫保护效力,我们在SPF鸡、普通肉鸡和蛋鸡上进行了免疫效力试验。应用这两种疫苗分别以103、104、105、l06PFu/羽颈部皮下接种1 日龄SPF鸡群,于7、lO、14、21日龄采血分离血清,测HI价。表达Ⅲ的重组鸡痘病毒免疫的鸡于lO日龄时可以测出HI价,】og 2,略低于常规疫苗。对于表达F的重组鸡痘病毒用间接ELISA和竞争ELISA方法检测抗体,结果表明这两种方法均能较好地检测出F抗体的消长规律。21日龄以105EL%的新城疫病毒NDVFtsEe攻击,连续观察lO天,结果103PFU/羽的接种剂量就足以保护SPF 鸡免受NDV强毒的攻击,与ND油苗、IV系苗相当。用肉鸡和蛋鸡进行的免疫效力试验,肉鸡在1日龄时进行免疫,21日龄时攻毒。蛋鸡在母源抗体下降到2 J092以下时免疫,,其保护率不高,表达酬基因的重组鸡痘病毒的保护效力略高于表达F基因的重组鸡痘病毒。初步分析其原因,可能是重组病毒疫苗的免疫效力受NDV和FPv母源抗体影响所致。在以上的免疫效力试验中同时辅以IFN—II作为免疫佐剂,经过比较可知,IFN可以降低重组鸡痘病毒对雏鸡增重的影响。综上所述,本室研制的表达NDVF聃E。株F、Ⅲ基因的重组鸡痘病毒在遗传稳定性及免疫保护效力方面达到《兽医生物制品检验规程》的标准,可望成为防制新城疫的一种新型疫苗。关键词:重组鸡痘病毒遗传稳定性免疫保护效力丁煽东:表达新城疫病毒F、HN基因重组鸡痘病毒疫苗的遗传稳定性和免疫效力试验3 ic stability and protective efficacy binant Fowlpox Virus ines Expressing Fand麟genes ofNewcastle disease virus : Advisots:Prof: Ding Weidong Liu Xiufan Wu Yantao College of Animal Science&Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China. Abstract Newcastle disease(ND)is one ofthe most important avian diseases that can cause severe economic losses in the poultry industry the world over+ Thecausative agent ofthedisease,Newcastle disease virus(NDV),is amember oftheParamyxovirus and contains anon—segmented negatire strand RNA genome. which encodes two envelop glycoproteins,the fusion(F)protein and the hemagglutinin-neuraminidase(雕) F protein is required for virus—ceIl fusion intheprocess ofinfection and hasbeen shown tobecapabIe ofinducing protective immunity to NDV,first in ina virus vector and more recentIF in afowlpox virus against Newcastle disease in current use include live ines consisting ofavirulent or attenuate strains of the virus and inactivated prising killed Virus from avirulent or pathogenic these ines have largely controlled the disease in the field,they inherit disadvantages of interfering serological surveillance or plete there iScurrently great interest inassessing thepossi