封闭群五指山小型猪血液生理生化指标的测定.pdf

2010广州一东莞首届国际小型猪学术论坛封闭群五指山小型猪血液生理生化指标的测定★闵凡贵,王希龙,袁文,张钰,潘金春,王静(广东省实验动物监测所,广州510260) 【摘要】目的对2~12月龄封闭群五指ll|小型猪的部分血液生理和生化指标进行测定和分析。方法常规方法测定五指山小型猪血液的19项生理和12项生化指标,统计分析各指标间的性别差异,并与近交系五指山小型猪以及其他小型猪的同类指标进行对比分析。结果封闭群五指山小型猪绝大多数血液生理、生化指标性别间差异不显著, 仅生理指标中的粒细胞分类计数和生化指标中的总胆崮醇差异显著。同近交系五指山小型猪同类指标比较,生理指标中血红蛋白和血小板相关指标存在差异,生化指标中有6项差异显著。同其他种类小型猪同类指标差异较与近交系五指山小型猪间差异明显。结论封闭群五指山小型猪血液生理、生化指标稳定,许多生理生化指标接近人类, 可能逐步替代犬、猴用于药物非临床安全评价和生物医学研究。【关键词】封闭群五指山小型猪:血液生理生化指标;对比分析 Determination ofSome Blood Physiological and Biochemical Parameters in Wuzhishan Mini-pigs ofClosed Colony MIN Fan—gui,WANG Xi-long,YUAN Wen,ZHANG Yu,PAN Jin-chun,WANG Jing (Guangdong Laboratory Animals Monitoring Institute,Guangzhou 510260,China) [Abstract] Objective The aim ofthis study was determine theblood physiological and biochemical parameters ofWuzhishan mini-pigs of closed colony aged 2—12 months Methods een physiological and twelve biochemical blood parameters Wuzhishan mini·pigs were measured and the differences between both sexes'between closed colony and inbred strain,between other strains were Among the blood physiological and biochemical indexes,only EO andTCHO showed significant difference between male and femalepigs main differences ofbloodphysiological indexes between closed colony and inbred strainfocused on indices ofhemoglobin and blood platelets,and there were differences sixdifferenti