EDC NHS 说明书.pdf

INSTRUCTIONS



EDC





22980 22981 77149 lecular-weight cutoff

Procedure
1. Equilibrate EDC to room temperature.
2. Add 2 mg of lyophilized BSA, OVA or KLH to 200 µl Conjugation Buffer. If using Pierce Imject® Carrier Proteins,
reconstitute using ultrapure water.
3. Dissolve up to 2 mg of the peptide or hapten in 500 µl of Conjugation Buffer and add it to the 200 µl carrier protein
solution.
4. For BSA or OVA conjugation, dissolve 10 mg of EDC in 1 ml of ultrapure water and immediately add 100 µl of this
solution to the carrier-peptide solution. For KLH conjugation, dissolve 10 mg of EDC in 1 ml of ultrapure water and
immediately add 50 µl of this solution to the carrier-peptide solution.
5. React for 2 hours at room temperature.
6. Purify the conjugate using a desalting column. If storing the immunogen for more than a few days, sterile filter the
conjugate and store in a sterile container at 4°C or -20°C.



Pierce Biotechnology PO Box 117 (815) 968-0747
3747 N. Meridian Road Rockford, lL 61105 USA (815) 968-7316 faxProcedure for Two-step Coupling of Proteins Using EDC and NHS or Sulfo-NHS
The following protocol, adapted from a procedure described by