维脑路通、精氨酸 对结肠吻合口愈合影响.docx

维脑路通、精氨酸_对结肠吻合口愈合影响examination were performed

1)Anastomotic bursting pressure measurement:The
4cm segment ofcolon wfls carefully cleared ,one side cormected to the
pressure test instrument and the other side connected to

injection pump
with

a
sutured
catheter,amethyleneblue solutionwasinfused

ata constant
rate
of2ml/min and at the￥fllTle time,cavity pressure was monitored。When leak of
solution occurred,the bursting pressure was recorded in mmHg
This parameter
indicates anastomotic mechanical strength

2)Anastomofic hydroxyproline
measurement:After anastomotic bursting pressure measurement,a lcm segment of
colon with the anastomotisis at the center was cut off 100mg in weigh,did
dehydration and ,add 6mmol/L hydrochloric acid

to

hydrolyzed for
12hours at 1 10"C,so that all amino acid peptide bond all ,after
oxidation,addedhydroxyprolinadisplayer(10%offdimeth'r'lamino-benzaldehyde
solution to the sampling to measure the content ofhydroxyproline using ultraviolet
spectrophotometer The ratio ofhydroxyproline in anization(Ixg/mg)was
adopted

as a

parameter

to
examme the results 3)Pathological
examination:The
segments ofanastomosis were fixed in 10%neutral formaldehyde,conventionally
embedded in paraffin,sliced 4am in thickness,after stained with hematoxylin and
eosin,examined macroscopically collagen cell and fiber pared the differences
between the groups
Results
A鼢the fourth postoperative

complete measures the pressure when
the anastomofie leakage was

not

observed in

any arginine

group,
venordton group and

the control group anastomotic bursting pressure respectively be
59 800=I:3 033mmHg,31 200-±2 588 mmHg and 25
200M
923 mmHg
Among the
three groups every two groups
ofbetween mutually
comparisons,there are significant
statistical difference(P<0
05):The
arginine group,venoruton group and the control