矽肺模型大鼠肺间质成纤维细胞mmp.doc

矽肺模型大鼠肺间质成纤维细胞MMP
作者:王海涛,刘昊,颜京霞,田艳霞,江小华,高俊玲【关键词】肺间质纤维化Expressinfatrixetallprteinase2and9inpulnaryfibrblastsfsilitifibrsisratdel 【Abstrat】AI…
作者:王海涛,刘昊,颜京霞,田艳霞,江小华,高俊玲
【关键词】肺间质纤维化
Expressin f atrix etallprteinase2 and 9 in pulnary fibrblasts f siliti fibrsis rat del
【Abstrat】 AI: T study the expressin f P2 and P9 in fibrblasts f rat siliti fibrsis del and its signifiane. ETHDS: Fifty pathgenfree istar rats ere randly divided int experiental grup and ntrl grup. Experiental grup as instillated ith Si2, hile ntrl grup ith nral saline. Pulnary interstitial fibrblasts ere islated n day 1, 3, 7, 14 and 28 after Si2 expsure. The prtein and RNA expressin f P2 as evaluated by iunytheistry and RTPR. RESULTS: ①The P2 prtein expressin in fibrblasts inreased n day 1 after Si2 expsure and reahed ties(t=,P ) that f ntrl grup n day 7. The expressin f P9 prtein als inreased n day 1 after Si2 expsure and reahed ties that f ntrl grup(t=, P )n day 7. ② The RNA expressin f P2 and P9 as enhaned in fibrblasts at 24 h after Si2 instillatin. NLUSIN: The expressin f P2 and P9 in fibrblasts is upregulated in siliti fibrsis del.
【Keyrds】 atrix etallprteinase; fibrblast ells; pulnary fibrsis; siliti fibrsis
【摘要】目的:探讨肺间质成纤维细胞P2和P9在Si2致肺纤维化中的表达及意义. 方法: 清洁级istar大鼠50只,随机分为对照组和实验组,实验组气管内注射Si2悬液,对照组代以等剂量生理盐水. 注射后1,3,7,14和28 d分离和提取肺间质成纤维细胞,应用免疫细胞化学和逆转录多聚酶链式反应(RTPR)方法,观