矽肺模型大鼠肺间质成纤维细胞MMP.doc

1 矽肺模型大鼠肺间质成纤维细胞 MMP 作者:王海涛,刘昊,颜京霞,田艳霞,江小华,高俊玲【关键词】肺间质纤维化 Expression of matrix metalloprotEinase2 and 9 in pulmonary fibroblasts of silicotic fibrosis rat model 【 Abstract 】 AIM: To study the expression of MMP2 and MMP9 in fibroblasts of rat silicotic fibrosis model and its significance. METHODS: Fifty pathogenfree Wistar rats were randomly divided into experimental group and control group. Experimental group was instillated with SiO2, while control group with normal saline. Pulmonary interstitial fibroblasts were isolated on day 1, 3, 7, 14 and 28 after SiO2 exposure. The protEIn and mRNA expression of MMP2 was evaluated by immunocytochemistry and RTPCR. RESULTS: ① The MMP2 protein expression in fibroblasts increased on day 1 after SiO2 exposure and reached times ( t= , ) that of 2 control group on day 7. The expression of MMP9 protein also increased on day 1 after SiO2 exposure and reached times that of control group ( t=, ) on day 7.② The mRNA expression of MMP2 and MMP9 was enhanced in fibroblasts at 24h after SiO2 instillation. CONCLUSION: The expression of MMP2 and MMP9 in fibroblasts is upregulated in silicotic fibrosis model. 【 Keywords 】 matrix metalloproteinase; fibroblast cells; pulmonary fibrosis; silicotic fibrosis 【摘要】目的: 探讨肺间质成纤维细胞 MMP2 和 MMP 9 在 SiO2 致肺纤维化中的表达及意义. 方法: 清洁级 Wista r 大鼠 50 只,随机分为对照组和实验组,实验组气管内注射 SiO2 悬液,对照组代以等剂量