【摘要】目的:建立高效液相色谱-电喷雾检测器(HPLC-CAD)法同时测定葡萄糖氯化钠注射液中葡萄糖、氯和钠的含量。方法:采用Thermo U3000-Corona CAD测定,用超纯水溶解葡萄糖氯化钠注射液,过滤后注入液相色谱仪测定。色谱柱为Thermo Mixed-Mode Hilic-1柱(3μm 21×150mm);柱温:40℃;流动相:50mmol/L醋酸铵和乙腈梯度洗脱;流速:03mL/min;进样体积:10μL。结果:葡萄糖的线性范围为005~100mg/mL(r=09993),氯和钠的线性范围为009~180mg/mL(r=09999)。方法回收率均>998%,精密度RSD均<20%,超纯水中ClO-、NO3-对葡萄糖氯化钠的含量测定几乎没有干扰。结论:该方法准确,快速、简便,可用于葡萄糖氯化钠注射液中葡萄糖、氯和钠的测定及其质量控制,对其类似化合物的测定具有参考价值。【关键词】葡萄糖氯化钠注射液;高效液相色谱;电喷雾检测器 【中图分类号】R917【文献标志码】 A【文章编号】1007-8517(2017)10-0018-03 Determination of Glucose,Cl- and Na+ in Glucose and Sodium Chloride Injectionby High Performance Liquid Chromatography with Charge Aerosol Detection(HPLC-CAD) SUN LiangguangGUO Zhenwang* Guangxi Wuzhou Institute for Food and Drug Control, Wuzhou 543001,China Abstract:Objective To establish a high performance liquid chromatographic with charge aerosol detection(HPLC-CAD) method for the determination of Glucose,Cl- and Na+ in Glucose and sodium chloride injection Methods An Thermo U-3000 HPLC system with a charged aerosol detector was usedThe Glucose and sodium chloride injection samples were dissolved by water and injected into a Thermo Mixed-Mode Hilic-1(3μm 21×150mm)column at 40℃The mobile phase posed of 50 mmol?L-1 ammonium acetate-acetonitrile with a flow rate of 03mL?min-1The injection volumn was 10μL Results The methodology recovery was higher than 99
