刀豆蛋白A 引起小鼠免疫性肝损伤机制研究.pdf

蚌埠医学院学报年月第卷第期
2011 5 36 5 445
[文章编号] ·基础医学·
1000鄄2200(2011)05鄄0445鄄03
刀豆蛋白引起小鼠免疫性肝损伤机制研究
A
姚晓敏,李宏伟,曲均革,崔相一
[摘要]目的:建立刀豆蛋白引起小鼠免疫性肝损伤模型并考察其损伤机制方法: 小鼠随机分为正常对照组
A(Con A) , 。 ICR
和模型组每组只模型组小鼠尾静脉注射- 1 - 1 对照组给予等体积生理盐水造模
Con A , 10 。 Con A Con A 2. 5 mg·ml ·kg , 。
后断头处死小鼠分别取肝脏和血液采用生化法测定小鼠血清丙氨酸氨基转移酶和天冬氨酸转移酶水平
8 h , , (ALT) (AST) ,
苏木精伊红染色法测定肝组织病理学改变法测定血清肿瘤坏死因子和干扰素含量结果:与
- ,ELISA 鄄琢(TNF鄄琢) 鄄酌(IFN鄄酌) 。
正常对照组比较模型组小鼠血清和水平均显著升高 P 肝细胞明显坏死伴炎细胞浸润同时血清
,Con A ALT AST ( < 0. 01), , ,
中和含量亦均显著升高 P 结论:尾静脉注射- 1 - 1 可建立免疫性肝炎模型其
TNF鄄琢 IFN鄄酌( < 0. 01)。 Con A 2. 5 mg·ml ·kg ,
损伤机制与炎症因子表达相关
。
[关键词] 免疫性肝损伤刀豆蛋白小鼠
; A;
[中国图书资料分类法分类号] [文献标识码]
R 575摇摇摇 A
The related mechanism of immunological liver injury induced by concanavalin A in mice
YAO Xiao鄄min,LI Hong鄄wei,QU Jun鄄ge,CUI Xiang鄄yi
Department of Pharmacy Zhejiang Pharmaceutical College Ningbo Zhejiang 315100 China
( , , , )
Abstract Objective Methods
[ ] :To establish the model of immunological liver injury in mice and investigate the related mechanisms. :
Twenty mice were randomly divided into normal control and Con A groups,10 in each. Mice of Con A group were injected with Con A
- 1 - 1
2. 5 mg·ml ·kg by vena caudalis,and sacrificed after 8 hours. The blood and liver tissue were collected to examine ALT and AST
levels spectrophotometrically,the histopathology changes were measured by HE method,and se