表没食子儿茶素没食子酸酯的甲基化分子修饰.pdf

※基础研究食品科学 2010, Vol. 31, No. 15 139
表没食子儿茶素没食子酸酯的甲基化分子修饰
吕海鹏,孙业良,林智* ,谭俊峰,郭丽
(中国农业科学院茶叶研究所,茶叶加工工程研究中心,浙江杭州 310008)
摘要:研究表没食子儿茶素没食子酸酯(EGCG)的甲基化分子修饰。以碘甲烷作为甲基供体,采用化学合成方法
研究 EGCG 的甲基化分子修饰,并通过 HPLC-MS 和 NMR 等对反应产物进行结构鉴定。结果表明:采用化学合
成方法能有效完成 EGCG 的甲基化分子修饰,并分离鉴定出 5 个 EGCG 甲基化衍生物,分别为 4″-Me-EGCG、
4′,4″-di-Me-EGCG、5,3′,4′,5′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,5′,
3″,4″,5″-octa-Me-EGCG。
关键词:表没食子儿茶素没食子酸酯;甲基化;分子修饰;碘甲烷;化学合成
Methylation of (-)-Epigallocatechin-3-gallate (EGCG)
..
LU Hai-peng,SUN Ye-liang,LIN Zhi*,TAN Jun-feng,GUO Li
(Engineering Research Center for Tea Processing, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou
310008, China)
Abstract :For EGCG methylation via chemosynthesis, methyl iodide was used as methyl donor and two reaction systems (
and ) were designed, in which the molar ratios between EGCG and methyl iodide were set at 1:80 and 1:1, respectively.
Reaction products TRI-1 and TRI-2 obtained from reaction system and , respectively were characterized by HPLC-
MS and NMR. The results showed that the molecular modification of EGCG could be essfully plished by the use of
chemosynthesis, and five EGCG derivatives, namely 4″-Me-EGCG, 4′,4″-di-Me-EGCG, 5,3′,4′,5′,3″,4″,5″-hepta- Me-
EGCG, 5,7,3′,4′,3″,4″,5″-hepta- Me-EGCG and 5,7,3′,4′,5′,3″,4″,5″-octa- Me-EGCG we