特应性皮炎患者皮损致病真菌定植的的分析.docx

2 目录英文缩略词表……………………………………………中文摘要…………………………………………………英文摘要…………………………………………………引言………………………………………………………材料与方法……………………………………………… 45679 结果……………………………………………………… 17 讨论……………………………………………………… 21 结论……………………………………………………… 24 参考文献………………………………………………… 25 附致综表…………………………………………………… 29 谢………………………………………………… 31 述…………………………………………………… 32 3 英文缩写英文全称中文全称 SDA , Sabouraud s dextrose agar 沙氏葡萄糖琼脂 AD Atopic dermatitis 特应性皮炎 SCORAD SCORing Atopic Dermatitis 特应性皮炎积分 VAS Visual analogue scales 视觉模拟评分法 tIgE Total immunoglobulin E 总免疫球蛋白 E sIgE Specific immunoglobulin E 特异性免疫球蛋白 E Candida albicans M Candida Chromogenic Medium 念珠菌显色培养基 MnSOD Mn superoxide dismutase 锰超氧化物歧化酶主要缩略词中英文对照(Abbreviation) 4 特应性皮炎患者皮损致病真菌定植分析硕士研究生:张秀钦导师:程波教授中文摘要目的:观察 50例 AD 患者念珠菌、红酵母、青霉、曲霉这 4种常见致敏真菌在 AD 患者皮肤表面定植情况,分析致敏真菌在 AD 发病中的作用。方法:研究对象为 2012 年6月至 2012 年12月间在福建医科大学附属第一医院皮肤科就诊的特应性皮炎患者共 50例,中度与重度患者各选 25例, 20例健康对照者,所有入选患者两周内均未使用抗真菌药、糖皮质激素或免疫抑制剂系统及外用治疗,分别对其皮损部位及非皮损部位采用常规真菌培养方法,将标本接种于沙保罗培养基,置 25℃恒温箱内培养;发现真菌及酵母样可疑菌落,转种沙保菌斜面,获得纯培养后鉴定, 根据菌落形态特征及颜色、菌落生长的快慢、镜下孢子及菌丝的特征鉴定。采用 SPSS 统计软件,用χ2检验分析阳性率差异的显著性, P< 为差异有统计学意义。结果: AD 患者皮肤表面念珠菌、红酵母检出率显著高于正常对照组,且重度患者检出率高于中度患者;青霉、曲霉检出率与正常对照组相比无统计学意义。结论: AD 患者皮肤表面念珠菌、红酵母定植明显高于正常对照组,并且与 AD 患者症状的严重程度相关。关键词:特应性皮炎,念珠菌,红酵母,真菌培养 5 Analysis of pathogenic fungal colonization on skin lesion of atopic dermatitis patients Postgraduate, Zhang Xiuqin Tutor, Prof . Cheng Bo Abstract Objective: To investigate the colonization of mon sensitizing fungi Candida, Rhodotorula, Penicillium, Aspergillus on the skin surface of AD patients, and to analyze sensitization fungi in the role of the AD. Methods: The study enrolled a total of50 patients with atopic dermatitis (moderate: n=25, severe : n=25) from June 2012 to December 2012 at Dermatology Department of the First Affiliated Hospital of Fujian Medical University, 20 healthy subjects served as controls. All enrolled patients did not use antifungal agents, glucocorticoids or immunosuppressants and topical treatment within two weeks before enrollment. Conve