酶催化柚皮苷水解.docx

酶催化柚皮苷水解
Applied Surface Science 257 (2022) 4096–4099
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e size,large sur-face area,pore volume,high adsorption capacity,and an ordered porous network for free diffusion of substrates and reaction prod-ucts [10].Those unique properties make it a potentially ideal matrix for enzyme [11,12]have appeared on the catalytic application of MCM-41as a catalyst support on the immobilization of a couple of enzymes and it showed good performance.
In the present study,naringinase was adsorbed onto MCM-41and crosslinked to silylated MCM-41by glutaraldehyde which provided a large number of aldehyde groups for the immobi-lized activity,thermal stability,reusability and kinetic mechanism of the immobilized naringinase were investigated and compared to the free ,immobilized naringinase was applied to hydrolyze naringin in the white grapefruits.
?Corresponding author.
E-mail address:pansy ******@ (). and methods
Naringinase (CAS Number 9068-31-9)from Penicillum decum-bens (activity:396U g ?1solid)and naringin (%)were obtained from Sigma Chemical Co (St Louis,MO,USA).Naringenin (HPLC,98%)and limonin (HPLC,98%)were from Chengdu Pus Bio-technology Co., MCM-41was from Shanghai Novel Chemical Technology Co