牛磺酸对大鼠脑缺血再灌注损伤的保护作用_0.doc

1 牛磺酸对大鼠脑缺血再灌注损伤的保护作用作者:成彩莲王晓娟何丽娅夏瑞明朱汉荣姚建华【关键词】, 脑保护作用【摘要】目的观察牛磺酸( Tau ) 对大鼠急性脑缺血再灌注损伤的保护作用。方法采用大鼠急性不完全性脑缺血再灌注损伤模型, 测定脑组织含水量、丙二醛( MDA )、超氧化物歧化酶( SOD )及谷胱甘肽过氧化物酶( GSH-Px ) 的含量。结果牛磺酸能显著对抗缺血再灌注大鼠脑组织含水量、 MDA 含量的升高( , ) 以及 SOD 、 GSH-P x 活性的降低( , )。结论牛磺酸具有保护脑缺血再灌注性损伤的作用, 机制与降低脑脂质过氧化和增强氧自由基清除酶的活性有关。关键词牛磺酸缺血再灌注损伤超氧化物歧化酶脑保护作用 Effects of taurine on acute ischemia reperfusional brain 2 lesion in rats 【 Abstract 】 Objective To observe the protective effects of taurine against acute brain lesion induced by is-chemia and reperfusional in The contents of water malondislehyde ( MDA ) and the activities of super-oxide dismutase ( SOD ) and glutathione peroxidase ( GSH-Px ) in the brain tissue of mice were measured on the cere-bral ischemia-reperfusion lesion model , which was produced by30minutes mon carotid artery ligation follow-ing60minutes and180minutes of reperfusion in Taurine significantly antagonized the increase of water and MDA contents ( , respectively ) and the attenuation of SOD and GSH-Px activities in the brain tissue of rats treated with acute ischemia-reperfusion ( , respectively ) .Conclusion Tau-rine has a protective effect against cerebral lesion by ischemia and is speculated that this protective ef-fect may be related to the inhibition of lipid peroxidation as well as the enhancement of SOD and GSH-Px activitives. Key words taurine cerebral reperfusion lesion superoxide 3 dismutase glutathione peroxidase 脑缺血再灌注损伤及药物的保护作用仍然是研究的热点,自由基和钙离子( Ca 2+ )在缺血性脑损伤中都占有重要地位。脑缺血经治疗后恢复血流供应, 发生缺血后再灌注,会产生大量自由基攻击细胞膜而造成细胞膜破裂,再次加重脑组织水肿和组织细胞坏死, 从而使临床症状加重并影响转归。牛磺酸( taurine , Tau )是广泛存在于人体内的一种β- 氨基酸,在神经、肌肉和腺体等可兴奋组织内含量都很高, 且有广泛的生理、药理学效应。有文献报道, Tau 对缺血心脏、肝脏及处于低氧状态的中枢神经系统均有保护作用[1~3]。本实验研究牛磺酸对大鼠脑缺血再灌注损伤的影响, 通过测定缺血再灌注大鼠脑组织中抗氧自由基酶 SOD 、 GSH-Px 的活性和 MDA 的含量来研究 Tau 的脑保护作用及其作用机制。 1 材料与方法 材料 实验动物健康 Wistar 大鼠(武汉科技大学实验中心动物室提供) 35 只,雌雄不限,体重( 250 ± 50)g。 4 主要试剂及仪器牛磺酸( Taurine , Tau , 进口分装, 武汉中健