人红细胞冰冻干燥保存研究的进展论文.doc

人红细胞冰冻干燥保存研究的进展论文.doc人红细胞冰冻干燥保存研究的进展论文
.freelan Red Blood Cells —— Revieethods of human red blood cells mainly include hypothermic storage (4℃) and cryopreservation (-80℃ or -196℃). The preservation time of hypothermic storage of red blood cells is relatively short and it is easy to be contaminated by microbes. Cryopreservation greatly prolongs the storage time,but it needs heavy storage equipments. Because the protective solutions in cryopreservation contain glycerol,red blood cells need plicated ove glycerol. These shortage methods limit their application to some special conditions,such as ethods of red blood cells,lyophilization has many advantages such as less temperature preservation,prone to be rehydrated. In this revieent of lyophilization of red blood cells,especially application of trehalose and its mechanism in the lyophilization of red blood cells atically discussed. This reviee theoretic guidance for developing a safe,simple and efficient preservation approach of red blood cells by lyophilization.
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红细胞是血液中含量最多的细胞,它通过其中的血红蛋白从肺部携带氧气运送到周边器官,在维持人体正常机能起着重要的作用。红细胞是一种高度分化的细胞,它没有细胞核、线粒体等复杂的细胞器,只有一层质膜包裹着血红蛋白。红细胞对于维持生命具有重要意义,人们由于创伤或其他原因造成大量失血而引起红细胞的丢失时,全身器官会氧气供应不足,从而危及生命。及时输注红细胞是治疗这类病例的有效手段。但是,目前在临床上使用的红细胞保存的主要方法有4℃低温和-80℃深低温保存。这些保存技术都有很好的效果,但又存在明显不足:4℃保存时间短,最长不过42天,而且红细胞容易受到污染,从而造成浪费;-80℃保存可以将保存时间延长至10-20年以上,但需要笨重的超低温冰箱等专门设备,运输不方便,能源消耗大。这些缺陷严重限制了常规保存方法在恶劣自然条件或战时条件下的应用[1]。对比之下,冰冻干