pbs缓冲液的配制方法.doc

PBS缓冲液配方不管是免疫组化,还是细胞培养中,常用到PBS缓冲液,下面是常用配方,供大家参考。用于细胞培养的PBS需含有氯化钾称取8gNaCl、、,溶于800ml蒸馏水中,,最后加蒸馏水定容至1L,灭菌即可。 8g,KCl ,Na2HPO4•12H2O , KH2PO4 ,溶于900ml双蒸水中,,加水定容至1L,常温保存备用。一般免疫组化用PBS,主要用来清洗,都只包含Na2HPO4•12H2O(磷酸氢二钠)和NaH2PO4•2H2O(磷酸二氢钠)两种组分,母液的配制:  Na2HPO4:称取  Na2HPO4-12H2O,溶于 1000ml  NaH2PO4:称取  NaH2PO4-2H2O,溶于1000ml 水各种浓度PB(pH=)的配制:先配  PB (pH=,100ml):取19ml  NaH2PO4, 81ml  的 Na2HPO4, 即可。然后  PB (pH=)按相应比例适当稀释 即可,如: PB(PH=) :取 500ml  PB,加水稀释至 1000ml 即可。 PB (PH=):取50ml  PB,加水稀释至 1000ml 即可。 PB (PH=):取100ml  M PB,加水稀释至 1000ml 即可。 若需要 NaCL 的话,加入 NaCL %(g/100ml)即可。 另:其它各种另 PH值的  PB(100ml)配方 :pH        NaH2PO4(ml)    Na2HPO4(ml)                                                    92                             8                         90                                                                                 85                                                                                                                                                                                                                                                                                    51