Akt途径对大鼠缺血再灌注心肌细胞凋亡的抑制.doc

葛根素通过PI3K/Akt途径对大鼠缺血再灌注心肌细胞凋亡的抑制
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作者:马亚飞刘新伟乔欣王群英杨海燕
【摘要】目的观察葛根素预处理对缺血再灌注(I/R)大鼠心肌细胞凋亡的影响及其与PI3K/Akt信号通路的关系。方法 30只SD大鼠随机分为假手术组(SH组)、缺血再灌注组(I/R组)、葛根素预处理组(PU组)、葛根素预处理+LY294002组(PU+ LY组)及缺血再灌注+LY294002组(I/R+ LY组)。除假手术组外,其他各组大鼠均行结扎冠状动脉前降支30 min,再灌注120 min,监测血流动力学,Western印迹法检测心肌组织pAkt信号蛋白的表达;TUNEL法检测心肌细胞凋亡。结果与I/R组比较,PU组能够明显改善心功能,抑制心肌细胞凋亡,并增加Akt的磷酸化水平(,)。结论葛根素预处理能够抑制缺血再灌注所致的心肌细胞凋亡,其作用机制与PI3K/Akt信号通路活化有关。
【关键词】葛根素;缺血再灌注;磷脂酰肌醇3激酶;丝(苏)氨酸激酶
【Abstract】 Objective To investigate the signaling pathway involved in the inhibition of apoptotic effect during myocardium ischemia reperfusion with Puerarin 30 male SD rats were divided randomly into five groups (n=6).The
myocardial injury of rats ware produced by the ligation of left coronary artery for 30 min followed by 120 min and±dp/dtmax were recorded at before ischemia, ischemia 30 min and reperfusion 120 min. The pAkt signaling protein expression was determined by Western blotting analysis. Cardiac myocyte apoptosis was determined by terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) methods. Results In puerarin preconditioning group, the systolic and diastolic function were improved; the apoptosis index was significantly attenuated; the expression of pAkt was increased pared with those of IR group (, P0