五步蛇毒降纤酶的快速分离与纯化l),刘国曹晓静,王秋红()—quan,LIUGuo—liang,CHAOXiao—jing,WANGQiu—hong(BiologicalproductsanddrugsinstituteofJinanMilitaryArea,Zhumadian,Henan,463000,China)[Abstract]—SepharoseFastFlowandsephadex75prepgradechromatagraphy,ponentsacorfespondi"gwiththenewnationalstandardswereobtained—ResultsNinty—twopercentsofhemorrhaginand6517fractionswithoutThromin—likeenzyme(TLE)activitywereeliminatedinthepreliminaryisolation,,,andupto1503Bu/mg,,thepurityoftheproductwashigh:SDS—Page:;HPIC:.[Keywords]venomofAgkisreonacuttts;defibrase;thrombin—likeenzyme(TIE);purifi—cation[摘要],采用EDTA络台,硫氰酸钾沉淀初步分离与快速离子交换和凝胶层析纯化相结台,%,非类凝血酶(TLE),,离子交换柱层析和凝胶过滤制得的降纤酶+其比活性太于达1503Bu/mg1200Bu/}每克五步蛇毒干粉可收获降纤酵2998116Bu;纯度选到SDS—PAGE电泳只在标准蛋白质分子量36000Da附近呈现单一电泳区带}HPLC单一峰蛄论建立r快速分离纯化降纤酶工艺方法,[关键词]塑;壁耋旦类凝血酶:纯化';硅[中围分类号]{[文献标识码]A[文章编号]1001—5639(2000)o3—0001—03降纤酶是一种安全有效的强力血栓溶解剂;具有蛇毒类凝血酶(Thrombin—likeenzyme,TIE)性质,即其在体外促凝,在体内实际上起着消耗纤维蛋白原的作用,故反而出现抗凝效果]主要用于各
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