第 30 卷，第 12 期 Vol. 30，No. 12 .1 mm, μm) chromatographic column was used, gradient elution was carried out with acetonitrile and 5 mmol／L ammonium acetate solution(containing %formic acid), the flow rate was mL／min, and injection volume was 10 μL. Multiple reaction monitoring(MRM) mode was used, chloramphenicol-D5 and metronidazole-D4 were used as internal standards and determined by ultra high performance liquid chromatography–tandem mass spectrometry. The mass concentrations of the four antibiotics in the range of –10 μg／L had good linear relat ithionship chromatographic w peak area, the linear correlation coefficients were more than , and the detection limit was μg／L. The relative standard deviation of the determination results was %–%(n=6), and the average recovery was %–%. The method is suitable for the determination of chloramphenicol，fosfomycin，fosfomycin and metronidazole in honey. Keywords ultra performance liquid chromatography tandem mass spectrometry; honey; chloramphenicol; thiamphenicol; sulfonamide; thiamphenicol; metronidazole 氯霉素、甲砜霉素和氟甲砜霉素属于氯霉素类 抗生素，对革兰氏阴性菌和革兰氏阳性菌具有很强 通信作者 李颜岩，硕士，副主任技师，研究方向为公共卫生理化检验 收稿日期 2021–09–30 引用格式
