水产品中氯霉素_甲砜霉素和氟甲砜霉素残留量高效液相色谱_串联2 2 2 2 2 文献识别码 A 文章编号 1000 7075 (2009) 02 0115 05 Determination of chloramphenicol , thiamphenicol and florfenicol residues in aquatic products by HPLC MS with internal standard method WAN G Zhi jie1 L EN G Kai liang1 SUN Wei hong1 L IU Yan ping2 ZHA I Yu xiu1 TAN Zhi jun1 GUO Meng meng1 WAN G Yu2 (1 Yellow Sea Fisheries Research Institute , Chinese Academy of Fishery Sciences , Qingdao 266071) (2 Shanghai Ocean University , 201306) ABSTRACT The met hod for t he determination of three chloramp henicols including chloram p henicol (CA P) ,thiamp henicol ( TA P) and florfenicol ( FF) in aquatic product s was developed by high performance liquid chromatograp hy tandem mass spectrometry ( HPL C MS/ MS) . Deu terium (d5 CA P) was used as internal standard instead of CA P , and was added to t he sample before extraction wit h ethyl acetate. The sample was extracted with et hyl acetate to transfer CA P , TA P and FF into the organic p hase. The extract liquid which consequently dried by blo wing wit h nitrogen was dissolved with water and defatted wit h hexane. The mass spectrometer was operated in t he negative ion mode using select
