克林霉素诱导实验在阳性球菌药敏实验中的应用.doc

克林霉素诱导实验在阳性球菌药敏实验中的应用.doc克林霉素诱导实验在阳性球菌药敏实验中的应用
【摘要】目的探讨阳性球菌中红霉素诱导克林霉素耐药性的发生情况,合理指导临床用药。方法 MIC法检测某院579株阳性球菌对克林霉素和红霉素的耐药情况;双纸片法检测可诱导克林霉素耐药。结果金黄色葡萄球菌、%、%、%。在红霉素耐药而克林霉素敏感的菌株中,%、%、%。在红霉素敏感株中未见克林霉素诱导性耐药。结论临床微生物实验室应加强可诱导克林霉素耐药性的检测,指导临床有效合理的使用抗生素。

【关键词】革兰阳性球菌;红霉素;克林霉素;D实验

The amplication of clisdamycin induction test in clinical microbiology susceptiability laboratories 【Abstract】 Objective To investigate the resistance of erthromycin and clidamycin in Gram-positive us and help to reasonably use clindamycin. Methods Use of MIC to detect 578 isolates randomly collected from January to September in our hospital, double antimicrobial agents detect the clidamycin induce Co-resistance to erythromycin and clindamycin accounted for %,%,% in us aureus,coagulase-negative us and beta-hemolylic the 129 eryRcliS isolates,the rate of inducible resistance to clindamycin (D-test positive) ycin induce test are The detection of inducible clindamycin resistance must be stressed in clinical microbiology laboratory to provide good support for rational antibiotic therapy.
【Key -positive us;erythromycin;clindamycin;D-test
大环内酯类抗生素作用于细菌核糖体50S大亚基,抑制细菌蛋白质合成,从而导致细菌死亡。靶位点的改变是葡萄球菌对大环内酯类、林可霉素类、链阳菌素B等抗生素获得性耐药的主要机制,并对MLSB(大环内酯类、林可霉素类、链阳菌素B等抗生素)抗生素表现为交叉耐药[1,2]。MLSB耐药分为固有耐药和诱导耐药。若诱导耐药则常规药敏实验表现为红霉素耐药而克林霉素敏感,用双纸片法