杨梅树皮中杨梅苷对照品制备及鉴定.pdf

杨梅树皮中杨梅菩对照品制备及鉴定
肖明徐祖疆王定勇
广东药学院药科学院广东广州 510006
摘要!目的!建立从杨梅树皮中制备杨梅普对照品的方法方法!采用聚酞胺柱层析和重结晶法对杨
梅树皮甲醇提取物进行分离纯化通过 UV IR MS 1~-NMR 和13C-NMR 进行结构鉴定通过 TLC 法
和~PLC法对对照品进行纯度检测结果!从杨梅树皮中分离纯化出的杨梅普对照品质量分数>
% 结论!该方法制备的杨梅普对照品符合中药化学对照品的相关要求可用于含黄酮类中药及其
相关制剂的质量控制
关键词!杨梅树皮杨梅普对照品药物制备
中图分类号! 文献标识码!A 文章编号!1673-2197 2013 08-0048-02
PreparationandidentificationofMyricitrinreferencesnbstancefromthebarkofMyricarubra
XiaoYue YuXiaoxia WangDingyong
SchoolofPharmacy GuangdongPharmaceuticalUniversity Guangzhou510006 China
Abstract Objective ToestablishamethodforpreparationofmyricitrinreferencesubstancefromthebarkofMSricarubra.
Methods MyricitrinwasisolatedandpurifiedfromtheMeO~-
raphyandre- IR MS 1~-NMRand13C--
tectedbythinlayerchromatography TLC andhighpressureliIuidchromatography ~PLC .Methods Myricitrinreferencesub-
%.resnlts Myricitrinreferencesubstancewasob-
%.Conclnsion Myricitrinreferencesubstancepreparedbythis
methodcansatisfythedemandsofChinesedrugchemicalreferencesubstancesandcouldbeusedintheIualitycontrolofChinese
drugmaterialscontainingflavonoidsanditspreparations.
KeyWords BarkofMyricarubra Myricitrin ReferenceSubstan